Generation of two iPSC lines derived from two unrelated patients with Gaucher disease

Abstract

Gaucher disease is the most common autosomal recessive lysosomal storage disorder, caused by mutations in the β-glucocerebrosidase gene GBA. Here we describe generation of iPSC from skin-derived fibroblasts from two unrelated individuals with neuronopathic forms of Gaucher disease. The donor for line iPSC-GBA-1, a 21 month old girl, carried the recurring GBA mutation c.1448 T > C, p.Leu483Pro at homozygous state; fibroblasts for line iPS-GBA-2 were obtained from a 4 year old girl compound heterozygous for the GBA mutations c.667 T > C, p.Trp223Arg and c.1226A > G, p.Asn409Ser. iPSCs were developed using integration free episomal vectors (OCT4, KLF4; L-MYC, SOX2 (OSKM) and LIN28).Resource tableUnlabelled TableUnique stem cell lines identifierHIHRSi001-AHIHRSi002-AAlternative names of stem cell linesiPSC-GBA-1 (HIHRSi001-A)iPSC-GBA-2 (HIHRSi002-A)InstitutionHertie Institute for Clinical Brain Research and German Center for Neurodegenerative Diseases (DZNE), Tübingen, GermanyContact information of distributorRebecca Schü[email protected] of cell linesInduced pluripotent stem cell (iPSC)OriginHumanCell sourceFibroblastsClonalityClonalMethod of reprogrammingNon-integrating episomal plasmidsMultiline rationaleTwo cell lines carrying individual GBA mutationsGene modificationYesType of modificationinherited mutationAssociated diseaseGaucher Disease, neuronopathic (OMIM # 230900, 23100)Gene/locusGBAiPSC-GBA-1: NM_000157.3(GBA): c.[1448 T > C]; [1448 T > C] | p.[Leu483Pro]; [Leu483Pro] (originally published as Leu444Pro (Tsuji et al., 1987))iPSC-GBA-2: NM_000157.3(GBA): c.[667 T > C];[1226A > G] | p.[Trp223Arg];[Asn409Ser] (Trp223Arg originally published as Trp184Arg (Choy et al., 2000))Method of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateJune 2017Cell line repository/bankN/AEthical approvalInstitutional Review Board (“Ethikkommission”) University of Tübingen Medical School, Germany, approval number 819/2016A (2016/12/21)