Sizing silver nanoparticles in biological matrices by spICP-MS

Abstract

Background: Advances in the characterization and determination of AgNPs in different matrices, investigations involving biological matrices such as urine, plasma, sweat, and saliva still need more studies for routine application. Aim: The present study aimed to investigate silver nanoparticles (AgNPs) sizing in human matrices (blood plasma, sweat, urine, and saliva) by Single Particle Inductively Coupled Plasma Mass Spectrometry (spICP-MS). The influence of sample dilution, NPs concentration, storage time were studied. Methods: Blood, plasma, urine and sweat were collected from healthy volunteers aged 20–30 years. The samples were then diluted 1:20 (urine, saliva and plasma) and 1:10 (sweat) in an aqueous solution of AgNPs. After the dilutions, the samples were analyzed by spICP-MS. At the end of the analyzes, the samples were stored at room temperature (25 °C), and another at -5 °C to evaluate the stability of the AgNPs. Results: Higher concentrations of AgNPs in solution caused interference of matrix compatibility due to Ag+ detection by spICP-MS. Storage time up to 144 h for AgNPs 2 µg L − 1 solutions in 1:20 diluted urine and water was suitable for AgNPs detection. Otherwise, AgNPs 2 µg L − 1 did not present a clear signal-background differentiation in a 1:20 plasma diluted solution after a storage time of 144 h. Conclusion: This study provided new knowledge for monitoring of AgNPs in biological samples, helping with the new regulations proposed by Environmental and Health Agencies.

Keywords

• Silver nanoparticles
• Urine
• Saliva
• Blood plasma
• Sweat
• Inductively coupled plasma mass spectrometry