Journal of Experimental Pharmacology (Oct 2020)
Hypoglycemic Activity of Curcuma mangga Val. Extract via Modulation of GLUT4 and PPAR-γ mRNA Expression in 3T3-L1 Adipocytes
Abstract
Dwiyati Pujimulyani,1 Wisnu Adi Yulianto,1 Astuti Setyowati,1 Seila Arumwardana,2 Hanna Sari Widya Kusuma,2 Ika Adhani Sholihah,2 Rizal Rizal,2,3 Wahyu Widowati,4 Ali Maruf5 1Faculty of Agroindustry, University of Mercu Buana Yogyakarta, Yogyakarta 55753, Indonesia; 2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung 40163, Indonesia; 3Biomedical Engineering Study Program, Department of Electrical Engineering, Faculty of Engineering, University of Indonesia, Jakarta 16424, Indonesia; 4Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia; 5Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Faculty of Medicine, Chongqing University, Chongqing 400030, People’s Republic of ChinaCorrespondence: Dwiyati Pujimulyani Email [email protected]: There would be over 600 million people living with diabetes by 2040 as predicted by the World Health Organization. Diabetes is characterized by raised blood sugar and insulin resistance. Insulin regulates the influx of glucose into the cell by upregulating the glucose transporter type 4 (GLUT4) expression on the plasma membrane. Besides, PPAR-γ also controls the metabolism of glucose in adipose tissues. Curcuma mangga Val., denoted as C. mangga, is a native Indonesian medicinal plant that has many beneficial effects, including an antidiabetic potential.Purpose: In this research, we aimed to disclose the hypoglycemic activity of ethanol extract of C. mangga (EECM) in 3T3-L1 fibroblasts-derived adipocyte cells in regulating glucose uptake as confirmed by the GLUT4 and PPAR-γ gene expression.Methods: The uptake of glucose was determined using radioactive glucose, while the gene expression of GLUT4, PPAR-γ, and β-actin was quantified using mRNA segregation and real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR).Results: We discovered that EECM interventions (200 and 50 μg/mL) increased glucose uptake in lipid-laden 3T3-L1 cells by 14.75 and 8.86 fold compared to the control non-insulin, respectively (p < 0.05). At the same doses, they also increased GLUT4 mRNA expression by 8.41 and 11.18 fold compared to the control non-insulin, respectively (p < 0.05). In contrast, EECM interventions (200 and 50 μg/mL) showed lower levels of PPAR-γ mRNA expression compared to the control metformin, indicating the anti-adipogenic potentials of EECM.Conclusion: EECM showed hypoglycemic activity in lipid-laden 3T3-L1 cells by improving glucose ingestion into the cells, which was mediated by increased GLUT4 expression and downregulated PPAR-γ expression.Keywords: 3T3-L1 adipocytes, Curcuma mangga Val, glucose uptake, GLUT4, PPAR-γ
