Bioactive Materials (Dec 2022)

Sustained delivery of rhMG53 promotes diabetic wound healing and hair follicle development

  • Hong Niu,
  • Haichang Li,
  • Ya Guan,
  • Xin Zhou,
  • Zhongguang Li,
  • Serana Li Zhao,
  • Peng Chen,
  • Tao Tan,
  • Hua Zhu,
  • Valerie Bergdall,
  • Xuehong Xu,
  • Jianjie Ma,
  • Jianjun Guan

Journal volume & issue
Vol. 18
pp. 104 – 115

Abstract

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MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53−/− mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.

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