Full Genome of Influenza A (H7N9) Virus Derived by Direct Sequencing without Culture

Abstract

An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient’s sputum without virus culture. The full genome showed substantial sequence heterogeneity and large differences compared with that from embryonated chicken eggs.

Keywords

• H7N9
• influenza A virus
• deep sequencing
• culture-free
• avian-origin
• direct sequencing