Nature Communications (May 2020)

Freeze-frame imaging of synaptic activity using SynTagMA

  • Alberto Perez-Alvarez,
  • Brenna C. Fearey,
  • Ryan J. O’Toole,
  • Wei Yang,
  • Ignacio Arganda-Carreras,
  • Paul J. Lamothe-Molina,
  • Benjamien Moeyaert,
  • Manuel A. Mohr,
  • Lauren C. Panzera,
  • Christian Schulze,
  • Eric R. Schreiter,
  • J. Simon Wiegert,
  • Christine E. Gee,
  • Michael B. Hoppa,
  • Thomas G. Oertner

DOI
https://doi.org/10.1038/s41467-020-16315-4
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 16

Abstract

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Calcium imaging has been used to visualize the activity of individual synapses, but cannot be scaled up to monitor thousands of synapses in tissue. Here, the authors present genetic tools that can be photoconverted from green to red to create a map of active synapses.