Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder

Kiymet Bozaoglu; Yujing Gao; Edouard Stanley; Miriam Fanjul-Fernández; Natasha J. Brown; Kate Pope; Cherie C. Green; Katerina Vlahos; Koula Sourris; Melanie Bahlo; Martin Delatycki; Ingrid Scheffer; Paul J. Lockhart

Stem Cell Research (Aug 2019)

Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder

Kiymet Bozaoglu,
Yujing Gao,
Edouard Stanley,
Miriam Fanjul-Fernández,
Natasha J. Brown,
Kate Pope,
Cherie C. Green,
Katerina Vlahos,
Koula Sourris,
Melanie Bahlo,
Martin Delatycki,
Ingrid Scheffer,
Paul J. Lockhart

Affiliations

Kiymet Bozaoglu: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia
Yujing Gao: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia
Edouard Stanley: Department of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia
Miriam Fanjul-Fernández: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia
Natasha J. Brown: Victorian Clinical Genetics Services, Victoria, Australia
Kate Pope: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia
Cherie C. Green: Olga Tennison Autism Research Centre, School of Psychology and Public Health, La Trobe University, Bundoora, Victoria, Australia; Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Katerina Vlahos: Murdoch Children's Research Institute, Parkville, Australia
Koula Sourris: Murdoch Children's Research Institute, Parkville, Australia
Melanie Bahlo: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, University of Melbourne, Melbourne, Australia
Martin Delatycki: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Victorian Clinical Genetics Services, Victoria, Australia
Ingrid Scheffer: Department of Paediatrics, University of Melbourne, Parkville, Australia; Department of Medicine, University of Melbourne, Austin Health, Melbourne, VIC, Australia; Florey Institute, Melbourne, VIC, Australia; Department of Neurology, Royal Children's Hospital, Melbourne, Australia
Paul J. Lockhart: Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia; Corresponding author at: Murdoch Children's Research Institute, 50 Flemington Road, Parkville, Victoria 3052, Australia.

Journal volume & issue: Vol. 39

Abstract

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We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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