Myeloperoxidase activity in an experimental model of metabolic syndrome

Abstract

Introduction: the metabolic syndrome is a state of chronic inflammation of low degree in which a set of metabolic alterations coexist. The increased activity of the enzyme myeloperoxidase is considered a sensitive marker of neutrophil infiltration in the inflammatory process. Objective: to determine the activity of the enzyme myeloperoxidase in the serum of Wistar rats with metabolic syndrome induced by the intake of 35% sucrose. Methods: an experimental study was carried out at the Department of Biomedical Experimental Research and the Experimental Toxicology Unit of the University of Medical Sciences of Villa Clara between September 2018 and May 2019. The universe was constituted by Wistar rats from 180 to 200g from the National Center for the Production of Laboratory Animals. We worked with a sample of two experimental groups of 10 male animals each, one subjected to a hyperglycemic diet that developed the metabolic syndrome and the other with healthy conventional food, all of them randomly formed, which were determined the myeloperoxidase in serum, as well as cholesterol, triglycerides and glycemia. Results: myeloperoxidase values in the metabolic syndrome induction group were significantly higher than the healthy one (2.57±0.33 vs 0.78±0.13). A moderate positive correlation of triacylglycerides and glycemia was observed with the activity of the myeloperoxidase enzyme. Cholesterol did not increase in the group with metabolic syndrome. Conclusions: myeloperoxidase activity is increased in animals with metabolic syndrome.

Keywords

• mieloperoxidasa
• síndrome metabólico
• ratas
• dieta hiperglucídica