INDICATORS OF CHEMILUMINESCENT ACTIVITY OF NEUTROPHILIC GRANULOCYTES IN HELICOBACTER PYLORI, ASSOCIATED STOMACH CANCER

Abstract

One of the leading and unresolved problems in medicine is gastric cancer. The risk of developing RJ in H. pylori-infected patients is 2–4 times higher than in uninfected patients. The role of the cells of the immune system in the transformation of chronic atrophic gastritis in the Russian pancreas is undeniable, while the first cells that respond to a foreign antigen are neutrophilic granulocytes (NG). The aim of our work was to study the chemiluminescent activity of neutrophilic granulocytes in Helicobacter pylori, associated gastric cancer. A survey of 50 patients with chronic atrophic gastritis (CAH) and 50 patients with gastric cancer (RG) was performed. The control group consisted of 85 healthy donors. Evaluation of spontaneous and induced chemiluminescence (CL) was carried out for 90 minutes on a 36-channel chemiluminescent analyzer “CL 3606” (Russia). The following indicators were determined: the time the curve reached the maximum CL intensity, the maximum value of the CL intensity, the area under the CL curve. As an amplifier used luminol. The respiratory burst inducer was the opsonized zymosan. The enhancement of CL induced by opsonized zymosan was evaluated by the ratio of the area induced to the area of spontaneous CL and indicated by the activation index. Statistical data processing was carried out using Statistica for Windows 8.0 application software packages. The critical level of significance in testing statistical hypotheses was taken to be p <0.05. In patients with pancreatic cancer, a decrease in the maximum intensity is revealed with spontaneous and induced CL of NG, which indicates a decrease in the functional activity of cells in malignant disease. In patients with pancreatic cancer, a decrease in the time to reach the maximum with spontaneous and induced CL of NG is recorded, which indicates a violation of the activation process of neutrophils. In patients with pancreatic cancer, the area under the curve increases with spontaneous and induced CL, a large operating time is revealed during a respiratory explosion of reactive oxygen species with low activity of the cells themselves. The most significant changes in the indicators of chemiluminescent activity of NG were detected in stage III pancreatic cancer, which probably indicates a depletion of the activity of immune system cells and the progression of the disease. The project “Development and implementation of a software package for screening and early diagnosis of gastric cancer by indicators of the immune, prooxidant and antioxidant systems to reduce mortality and disability” was supported by the Krasnoyarsk Regional Science Fund.

Keywords

• хемилюминесцентная активность
• нейтрофильные гранулоциты
• хронический атрофический гастрит
• h. pylori
• рак желудка