Fabrication of multiwalled carbon nanotubes/carrageenan-chitosan@ Ce and Sr substituted hydroxyapatite biocomposite coating on titanium: In vivo bone formation evaluations

Abstract

Mineral substituted hydroxyapatite (MHAp) is one of the most significant bony mineral parts that have been broadly utilized as bony substitution matters as a result of its cytocompatible and bioactive properties. Though, the utilization of hydroxyapatite as bony inserts is confined because of its poor mechanical and fragile properties. To conquer this deformity and to produce appropriate bony embed substance; hydroxyapatite is joined with biocompatible macromoles (kappa-Carrageenan (KCG) and chitosan (CTS)). To improve the mechanical strength of the hydroxyapatite based biocomposite, functionalized multiwalled carbon nanotubes (FMWCNT) is joined to the biocomposite, which has for quite some time been considered for hard and delicate tissue implant because of its remarkable auxiliary and mechanical characteristic properties. It is outstanding that FMWCNT platform is the most-conspicuous substance for the hard tissues reproduction. We have developed a novel FMWCNT/KCG@MHAp nanocomposite on titanium (Ti) implant. The developed nanocomposite coatings implants were portrayed by different strategies (SEM, XRD, TEM). The outcomes show the arrangement and homogeneous conveyance of constituents in the nanocomposite coatings. Addition of FMWCNT and KCG into the MHAp nanocomposite altogether improves the attachment quality and flexible modulus of the scaffolds. Moreover, the reactions of mesenchymal stem cells (MSCs) culture onto the nanocomposite coating show that the suitability of cells was significantly high for FMWCNT and KCG consolidated MHAp than for MHAp. Animal model examinations demonstrate the nearness of delicate osteoblast and fibrous tissue development with no noteworthy provocative symptoms, which recommends that the Addition of FMWCNT and KCG encourage the great biochemical role of MHAp. For load-bearing applications, examine in different bony models is expected to substantiate the medical accessibility. In this manner, from the acquired outcomes, we propose that FMWCNT/KCG@MHAp nanocomposite can be considered as a prospective possibility for orthopedic applications. Keywords: Carrageenan, Chitosan, Carbon nanaotubes, Hydroxyapatite, Nanocomposite, Titanium