Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells

Han Wang; Moran Hu; Zhaoxue Ding; Xiaolong Zhou; Songbai Yang; Zhonghao Shen; Feifei Yan; Ayong Zhao

Poultry Science (May 2022)

Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells

Han Wang,
Moran Hu,
Zhaoxue Ding,
Xiaolong Zhou,
Songbai Yang,
Zhonghao Shen,
Feifei Yan,
Ayong Zhao

Affiliations

Han Wang: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Moran Hu: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Zhaoxue Ding: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Xiaolong Zhou: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Songbai Yang: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Zhonghao Shen: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Feifei Yan: Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China
Ayong Zhao: Corresponding author:; Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, China

Journal volume & issue: Vol. 101, no. 5
p. 101805

Abstract

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ABSTRACT: Phosphoglycerate dehydrogenase (PHGDH) is the rate-limiting enzyme in the serine synthesis pathway. However, the regulatory role of PHGDH in muscle development is unclear. We report that the expression of PHGDH increased significantly during proliferation of chicken skeletal muscle satellite cells. Knockdown of PHGDH by an siRNA suppressed myoblast proliferation, whereas overexpression of PHGDH enhanced muscle cell proliferation. Furthermore, PHGDH promoted the expression of Forkhead box protein M1 (FoxM1). Knockdown of FoxM1 by an siRNA attenuated the proliferation of chicken muscle cells, whereas its overexpression significantly promoted proliferation. Additionally, siRNA-PHGDH inhibited pcDNA3.1-FoxM1-induced FoxM1 expression in chicken muscle cells. Moreover, PHGDH inhibition overcame the stimulation by pcDNA3.1-FoxM1 of cell cycle-related gene expression. We propose that PHGDH accelerates chicken muscle cell proliferation by increasing FoxM1 expression.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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