A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis

Nicole D. Barth; Ramon Subiros-Funosas; Lorena Mendive-Tapia; Rodger Duffin; Mario A. Shields; Jennifer A. Cartwright; Sónia Troeira Henriques; Jesus Sot; Felix M. Goñi; Rodolfo Lavilla; John A. Marwick; Sonja Vermeren; Adriano G. Rossi; Mikala Egeblad; Ian Dransfield; Marc Vendrell

doi:10.1038/s41467-020-17772-7

Nature Communications (Aug 2020)

A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis

Nicole D. Barth,
Ramon Subiros-Funosas,
Lorena Mendive-Tapia,
Rodger Duffin,
Mario A. Shields,
Jennifer A. Cartwright,
Sónia Troeira Henriques,
Jesus Sot,
Felix M. Goñi,
Rodolfo Lavilla,
John A. Marwick,
Sonja Vermeren,
Adriano G. Rossi,
Mikala Egeblad,
Ian Dransfield,
Marc Vendrell

Affiliations

Nicole D. Barth: Centre for Inflammation Research, University of Edinburgh
Ramon Subiros-Funosas: Centre for Inflammation Research, University of Edinburgh
Lorena Mendive-Tapia: Centre for Inflammation Research, University of Edinburgh
Rodger Duffin: Centre for Inflammation Research, University of Edinburgh
Mario A. Shields: Cold Spring Harbor Laboratory
Jennifer A. Cartwright: Centre for Inflammation Research, University of Edinburgh
Sónia Troeira Henriques: Institute for Molecular Bioscience, The University of Queensland
Jesus Sot: Instituto Biofisika (CSIC, UPV/EHU) and Departamento de Bioquímica, Universidad del País Vasco
Felix M. Goñi: Instituto Biofisika (CSIC, UPV/EHU) and Departamento de Bioquímica, Universidad del País Vasco
Rodolfo Lavilla: Laboratory of Medicinal Chemistry and Institute of Biomedicine U. Barcelona (IBUB), Faculty of Pharmacy, University of Barcelona
John A. Marwick: Centre for Inflammation Research, University of Edinburgh
Sonja Vermeren: Centre for Inflammation Research, University of Edinburgh
Adriano G. Rossi: Centre for Inflammation Research, University of Edinburgh
Mikala Egeblad: Cold Spring Harbor Laboratory
Ian Dransfield: Centre for Inflammation Research, University of Edinburgh
Marc Vendrell: Centre for Inflammation Research, University of Edinburgh

DOI: https://doi.org/10.1038/s41467-020-17772-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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Programmed cell death or apoptosis is an essential biological process that is impaired in some diseases and can be used to assess the effectiveness of drugs. Here the authors design Apo-15 as a fluorogenic peptide for the detection and real-time imaging of apoptotic cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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