Disturbance of the expression of circadian genes Per1, Clock and BMal1 in rat liver, lung, testis, kidney and heart under silver nanoparticles action on organism

Abstract

Most of physiological and biochemical processes in living organisms have circa­dian character. Disturbance of the regulation of circadian processes leads to developing different pathological processes and cancer, in particular. In this work, we have studied the effect of silver nanoparticles on the expression of very important regulatory genes which control the circadian processes: Per1, Clock and BMal1. We have shown that the level of Per1 expression significantly increased in the liver and lung but decreased in the testes. The level of Clock mRNA expression also increased in liver and lung but decreased in kidney. The Clock mRNA expression in testis and heart was observed at late period of silver nanoparticles action. Thus, the expression of Per1, Clock and BMal1 mRNA significantly changed in tissue specific manner in rats treated with silver nanoparticles. Disturbance of these genes expression can destroy cellular signal pathways and leads to developing pathological processes. The results of our investigation clearly demonstrated that silver nanoparticles have significant effect on the regulatory mechanisms which control cell metabolism via circadian gene expression. Expression of circadian genes can be a sensitive test for the detection of silver nanoparticles action on the organisms.

Keywords

• per1
• clock
• bmal1
• silver nanoparticles
• liver
• lung
• testes
• rats