Generation and characterization of human induced pluripotent stem cells (iPSCs) from three individuals without age-related macular degeneration

Louet Koolen; Giuliana Gagliardi; Sofie C.A. ten Brink; Anita de Breuk; Thomas J. Heesterbeek; Carel B. Hoyng; Silvia Albert; Anneke I. den Hollander

Stem Cell Research (Apr 2022)

Generation and characterization of human induced pluripotent stem cells (iPSCs) from three individuals without age-related macular degeneration

Louet Koolen,
Giuliana Gagliardi,
Sofie C.A. ten Brink,
Anita de Breuk,
Thomas J. Heesterbeek,
Carel B. Hoyng,
Silvia Albert,
Anneke I. den Hollander

Affiliations

Louet Koolen: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands; Corresponding author.
Giuliana Gagliardi: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands
Sofie C.A. ten Brink: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands
Anita de Breuk: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands
Thomas J. Heesterbeek: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands
Carel B. Hoyng: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands
Silvia Albert: Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands
Anneke I. den Hollander: Department of Ophthalmology, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, Nijmegen, the Netherlands; Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands

Journal volume & issue: Vol. 60
p. 102670

Abstract

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Age-related macular degeneration (AMD) is a major cause of vision loss among the elderly in the Western world. AMD is multifactorial eye disease with a strong genetic contribution. Here, we report the generation and characterization of induced pluripotent stem cells (iPSCs) derived from peripheral blood mononuclear cells of three individuals above 70 years of age without AMD. These cell lines were generated to serve as control lines for cellular studies investigating the disease mechanisms and developing therapeutic interventions for AMD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal