Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses

Guoxing Luo; Yuanjun Zeng; Han Yang; Yijian Li; Lianwei Yang; Cao Li; Feibo Song; Shiyin Zhang; Tingdong Li; Shengxiang Ge; Jun Zhang; Ningshao Xia

iScience (Oct 2022)

Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses

Guoxing Luo,
Yuanjun Zeng,
Han Yang,
Yijian Li,
Lianwei Yang,
Cao Li,
Feibo Song,
Shiyin Zhang,
Tingdong Li,
Shengxiang Ge,
Jun Zhang,
Ningshao Xia

Affiliations

Guoxing Luo: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Yuanjun Zeng: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Han Yang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Yijian Li: College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Lianwei Yang: R&D Department of Xiamen Innodx Biotechnology Co., Ltd, Xiamen 361000, China
Cao Li: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Feibo Song: National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, China
Shiyin Zhang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Tingdong Li: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; Corresponding author
Shengxiang Ge: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; Corresponding author
Jun Zhang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
Ningshao Xia: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, China

Journal volume & issue: Vol. 25, no. 10
p. 105099

Abstract

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Summary: Non-replicating rotavirus vaccines are an alternative strategy to improve the efficacy and safety of rotavirus vaccines. The spike protein VP4, which could be enzymatically cleaved into VP8∗ and VP5∗, is an ideal target for the development of recombinant rotavirus vaccine. In our previous studies, we demonstrated that the truncated VP4 (aa26-476, VP4∗) could be a more viable vaccine candidate compared to VP8∗ and VP5∗. Here, to develop a human rotavirus vaccine, the VP4∗ proteins of P[4], P[6], and P[8] genotype rotaviruses were expressed. All VP4∗ proteins can stimulate high levels of neutralizing antibodies in both guinea pigs and rabbits when formulated in aluminum adjuvant. Furthermore, bivalent VP4∗-based vaccine (P[8] + P[6]-VP4∗) can stimulate high levels of neutralizing antibodies against various genotypes of rotavirus with no significant difference as compared to the trivalent vaccines. Therefore, bivalent VP4∗ has the potential to be a viable rotavirus vaccine candidate for further development.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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