Single-nuclei RNA-seq on human retinal tissue provides improved transcriptome profiling

Qingnan Liang; Rachayata Dharmat; Leah Owen; Akbar Shakoor; Yumei Li; Sangbae Kim; Albert Vitale; Ivana Kim; Denise Morgan; Shaoheng Liang; Nathaniel Wu; Ken Chen; Margaret M. DeAngelis; Rui Chen

doi:10.1038/s41467-019-12917-9

Nature Communications (Dec 2019)

Single-nuclei RNA-seq on human retinal tissue provides improved transcriptome profiling

Qingnan Liang,
Rachayata Dharmat,
Leah Owen,
Akbar Shakoor,
Yumei Li,
Sangbae Kim,
Albert Vitale,
Ivana Kim,
Denise Morgan,
Shaoheng Liang,
Nathaniel Wu,
Ken Chen,
Margaret M. DeAngelis,
Rui Chen

Affiliations

Qingnan Liang: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine
Rachayata Dharmat: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine
Leah Owen: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Akbar Shakoor: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Yumei Li: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine
Sangbae Kim: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine
Albert Vitale: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Ivana Kim: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Denise Morgan: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Shaoheng Liang: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Nathaniel Wu: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine
Ken Chen: Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center
Margaret M. DeAngelis: Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine
Rui Chen: HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine

DOI: https://doi.org/10.1038/s41467-019-12917-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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The retina is a heterogeneous tissue composed of multiple cell types. Via single-nuclei RNA sequencing on human neural retinal tissue, the authors characterise the transcriptome profile for individual cell types in the human retina.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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