Structural basis of Q-dependent transcription antitermination

Jing Shi; Xiang Gao; Tongguan Tian; Zhaoyang Yu; Bo Gao; Aijia Wen; Linlin You; Shenghai Chang; Xing Zhang; Yu Zhang; Yu Feng

doi:10.1038/s41467-019-10958-8

Nature Communications (Jul 2019)

Structural basis of Q-dependent transcription antitermination

Jing Shi,
Xiang Gao,
Tongguan Tian,
Zhaoyang Yu,
Bo Gao,
Aijia Wen,
Linlin You,
Shenghai Chang,
Xing Zhang,
Yu Zhang,
Yu Feng

Affiliations

Jing Shi: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Xiang Gao: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Tongguan Tian: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Zhaoyang Yu: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Bo Gao: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Aijia Wen: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Linlin You: Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences
Shenghai Chang: Center of Cryo Electron Microscopy, Zhejiang University School of Medicine
Xing Zhang: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Yu Zhang: Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences
Yu Feng: Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine

DOI: https://doi.org/10.1038/s41467-019-10958-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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Bacteriophage Q protein serves as a model regulator for the study of transcription elongation. Here the authors report a cryo-EM structure of an intact Q-engaged arrested complex, revealing the interactions responsible for σ-dependent pause, Q engagement, and Q-mediated transcription antitermination.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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