Blood serum 48 kDa form of unconventional myosin 1c characterizes the early stage of multiple sclerosis

Abstract

In accordance with the modern ideas of multiple sclerosis (MS) B-lymphocytes play a significant role in the pathological process development. In this regard, it is relevant to search for new biomarkers of B-lymphocytic origin, which can reflect the clinical features of this disease. Aim of study – to assess the relationships between the level of blood serum 48 kDa form of unconventional myosin 1c (48 kDa Myo1c) in patients with multiple sclerosis and stage of this disease, its severity and type. Materials and methods. 1 ml of blood serum was diluted 2-fold with phosphate buffer saline and then trichloroacetic acid (TCA) was added to 10 % of final concentration. The supernatant containing TCA-soluble compounds was isolated and mixed with acetone. Then, centrifugation and electrophoresis in the presence of sodium dodecylsulphate were performed. The 48 kDa Myo1c was identified by its molecular weight comparing after Coomassie Brillant Blue G staining of gel and Western blot analysis using polyclonal anti-Myo1c rabbit antibodies. Results. The level of the 48 kDa Myo1c was significantly higher in the MS patients compared with that in healthy controls. The disease duration was shorter in patients with high level of the 48 kDa Myo1c, compared to patients with low level of the 48 kDa Myo1c. High level of the 48 kDa Myo1c was associated with a relapsing-remitting MS, while low level – with secondary progressive type of the disease. In the group with the low 48 kDa Myo1c level a disability rate was significantly higher, unlike in patients with the medium level of 48 kDa Myo1c. Conclusions. An increased blood serum level of the 48 kDa Myo 1c in MS patients is combined with the early stage of the MS when its diagnostics is the most complicated.

Keywords

• multiple sclerosis
• biomarkers
• unconventional myosin 1c
• clinical course
• early stage