Sequence terminus dependent PCR for site-specific mutation and modification detection

Gaolian Xu; Hao Yang; Jiani Qiu; Julien Reboud; Linqing Zhen; Wei Ren; Hong Xu; Jonathan M. Cooper; Hongchen Gu

doi:10.1038/s41467-023-36884-4

Nature Communications (Mar 2023)

Sequence terminus dependent PCR for site-specific mutation and modification detection

Gaolian Xu,
Hao Yang,
Jiani Qiu,
Julien Reboud,
Linqing Zhen,
Wei Ren,
Hong Xu,
Jonathan M. Cooper,
Hongchen Gu

Affiliations

Gaolian Xu: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Hao Yang: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Jiani Qiu: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Julien Reboud: Division of Biomedical Engineering, University of Glasgow
Linqing Zhen: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Wei Ren: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Hong Xu: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University
Jonathan M. Cooper: Division of Biomedical Engineering, University of Glasgow
Hongchen Gu: School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University

DOI: https://doi.org/10.1038/s41467-023-36884-4
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 11

Abstract

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Rapid and facile detection of specific nucleic acid modifications could have numerous applications. Here the authors present Specific Terminal Mediated Polymerase Chain Reaction (STEM-PCR) as a generic and accessible approach, and demonstrate proof-of-principle cancer biomarker detection.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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