A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Lun Cui; Antoine Vigouroux; François Rousset; Hugo Varet; Varun Khanna; David Bikard

doi:10.1038/s41467-018-04209-5

Nature Communications (May 2018)

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Lun Cui,
Antoine Vigouroux,
François Rousset,
Hugo Varet,
Varun Khanna,
David Bikard

Affiliations

Lun Cui: Synthetic Biology Group, Department of Microbiology, Institut Pasteur
Antoine Vigouroux: Synthetic Biology Group, Department of Microbiology, Institut Pasteur
François Rousset: Synthetic Biology Group, Department of Microbiology, Institut Pasteur
Hugo Varet: Hub Bioinformatique et Biostatistique, Institut Pasteur - C3BI, USR 3756 IP CNRS
Varun Khanna: Hub Bioinformatique et Biostatistique, Institut Pasteur - C3BI, USR 3756 IP CNRS
David Bikard: Synthetic Biology Group, Department of Microbiology, Institut Pasteur

DOI: https://doi.org/10.1038/s41467-018-04209-5
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

CRISPR interference (CRISPRi) is a method for targeted silencing of transcription that requires the coexpression of protein dCas9 and a customized guide RNA. Here, Cui et al. show that certain guide RNAs induce toxicity in E. coli, and provide design rules to minimize off-target effects.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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