THE EFFICIENCY OF USING ALGA EXTRACTS IN THE MICROCLONAL REPRODUCTION OF SOME PLANT SPECIES

Abstract

Aim: to study the development of explants of some cultivated plants in vitro conditions using MC medium with addition of algae extracts. Materials and methods: the standard Murashige and Skoog nutrient medium (1962) with a half content of salts was used in our work. There were used several control options: 1) MC medium without additives; 2) medium with addition of 0.1 mg/l of artificial hormones – BAP (benzylaminopurine); 3) 0.1 mg/l GA (gibberelic acid); 4) 0.1 mg/l of IBA (indolylbutyric acid) after autoclaving. In the experimental variants, algae CeramiumvirgatumRoth., Ulvaintestinalis L., Cladophoravagabunda (L.) Hoek., Ulvacompressa L. were introduced into a nutrient medium in 5 and 10% (V/V) concentrationsafter its autoclaving. The effect was determined by the change of the growth indicators. Results: water extracts of some species of algae and their biological activitywere studied. The impact of extracts on the development of explants of some cultivated plants in vitro conditions was determined. It was shown that the strength and direction of changescaused by the extracts depended on algae species. The overdose with mineral substances was observedwith the increase of concentration of some algae extracts in nutrient medium. It is reasonable to use extracts at the level of microelements. Conclusions: synthetic nutrient medium can be supplemented with algae extracts, it will cause increasing of the quantity of explants (clones).

Keywords

• біологічно активні речовини
• водорості
• екстракт
• ростові показники