Determination of von Willebrand factor level in patient with sickle cell diseasein vaso‐occlusive crisis

Abstract

Abstract Background Sickle cell anemia (SCA) is a hypercoagulable state characterized by a significant alteration in hemostatic parameters that may predispose to increased risk of vaso‐occlusive crisis (VOC). The role of von Willebrand factor (VWF) in the pathogenesis of VOC has not been fully investigated in Nigeria. Objective The objective of this study was to evaluate the level of VWF in subjects with sickle cell disease (SCD) in Calabar, Nigeria, to determine its role in the pathogenesis of VOC. Methods This was a comparative study carried out at the University of Calabar Teaching Hospital, Calabar, Nigeria. Sixty patients with SCA in VOC and 50 healthy controls were included. VWF levels were measured using Assaypro enzyme‐linked immunosorbent assay kits. Results The mean age of patients with SCA in VOC and controls was 23.5 ± 7.2 years and 26.5 ± 5.6 years, respectively. The means (standard deviations) of VWF in patients in VOC and controls were 2.52 ± 0.34 IU/mL and 1.41 ± 0.23 IU/mL, respectively. There was no correlation of hematocrit and VWF in VOC (r = −0.034; P = .80), while there was a modest inverse correlation in controls. Conclusions Levels of VWFare elevated in a VOC state and thus may be implicated in the pathogenesis of VOC.

Keywords

• sickle cell anemia
• vaso‐occlusive crisis
• von Willebrand factor