Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice

Linlin Wang; Mingluo Pan; Dongyao Li; Yiting Yin; Tian Jiang; Shuguang Fang; Gang Wang; Jianxin Zhao; Hao Zhang; Wei Chen

Journal of Functional Foods (Nov 2017)

Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice

Linlin Wang,
Mingluo Pan,
Dongyao Li,
Yiting Yin,
Tian Jiang,
Shuguang Fang,
Gang Wang,
Jianxin Zhao,
Hao Zhang,
Wei Chen

Affiliations

Linlin Wang: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China
Mingluo Pan: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China
Dongyao Li: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China
Yiting Yin: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China
Tian Jiang: Jiangsu Wecare Biotechnology Co., Ltd, Wujiang 215200, PR China
Shuguang Fang: Jiangsu Wecare Biotechnology Co., Ltd, Wujiang 215200, PR China
Gang Wang: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Corresponding authors at: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
Jianxin Zhao: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China
Hao Zhang: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Corresponding authors at: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
Wei Chen: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, PR China

Journal volume & issue: Vol. 38
pp. 486 – 496

Abstract

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The aim of this study was to evaluate the effects of different kinds of oligosaccharides on cecal microbiota. These oligosaccharides were a fructo-oligosaccharide (FOS) formulation consisting of 95% FOS (FOS95), a galacto-oligosaccharide (GOS) formulation consisting of 90% GOS (GOS90), and an isomalto-oligosaccharide (IMO) formulation consisting of 90% IMO (IMO90), at dosages of 0.8, 4, and 8 g/d/kg bw. FOS95, IMO90, and GOS90 were each gavaged to BALB/c mice for 14 days. Sequencing of 16S rDNA amplicons showed that the abundance of Actinobacteria and TM7 dramatically decreased in constipation group. Feeding constipated mice with FOS95, IMO90, and GOS90 resulted in decreased ratio of Firmicutes to Bacteroidetes, and increased abundances of Bifidobacterium, Bacteroides, Rikenella, Lactobacillus, and Clostridium and decreased abundances of Oscillospira, Coprococcus, and Dorea at the genus level. Overall, our results demonstrate that FOS95, IMO90, and GOS90 modulate the cecal microbiome in constipated mice.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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