Purification of phospholipids recovered from thin-layer chromatograms for infrared spectral analysis

Abstract

An application of the Folch washing procedure for the removal of contaminants from phospholipid classes recovered from thin-layer chromatograms is described. The infrared spectra from the purified phospholipids are satisfactory for identification. The pattern of the major milk phospholipids separated on a thin-layer plate is presented.

Keywords

• thin-layer chromatogram
• recovered phospholipids
• purification
• Folch wash
• infrared spectra
• milk