Sealing ability of mineral trioxide aggregate, Biodentine, and EndoSequence RRM putty used as retrograde restorative material: An in vitro bacterial leakage model study

Abstract

Aim: the present study aimed to evaluate and compare the bacterial sealing ability of ProrootMTA, Biodentine and EndoSequenceRRM putty as retrograde filling material using E-faecalis leakage model over a period of 6weeks. Materials and Method: Forty single rooted human premolar teeth were collected, disinfected and decoronated to a standardized of 16mm. Cleaning and shaping was done up to 40#K-file apical preparation and step back method. The roots were sectioned 3 mm from the apex. Root end cavities were prepared using ultrasonic tips (Woodpecker EMS EndoScaler TipE3D) and restored using the three different materials. Specimens were divided in to four groups with a sample size of n =10. Group 1–ProRootMTA, Group2– Biodentine, Group3– EndoSequenceRRM putty, Group 4-Control without any filling. Specimens were then mounted on the E-faecalis (ATCCstrain29212) bacterial leakage models. The bacterial leakage was assessed by measuring the optical density of the broth at every 7th day for a 6 weeks period. Results: In the baseline reading there was significant difference between all the groups. In the 2nd week, Group1 has got significantly higher values than Group 3 and not with Group 2. In the 3rd, 4th, 5th and 6th weeks, Group1 show significantly higher turbidity values than Group 2 and 3. No significant difference between Group2 and 3. Conclusion: Within limitations of this study it was concluded that, both ESRRMputty and Biodentine have got comparable sealing properties. ProrootMTA has got higher microleakage than others in the 3rd, 4th, 5th and 6th week. The microleakage for ProrootMTA, Biodentine and ESRRM putty was increasing up to the third week following which it is static.

Keywords

• proroot mta
• biodentine
• endosequence rrm putty
• bacterial sealing
• retrogarde restorations