Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Afrah Nawaz; Syed Mohsin Ali; Nosheen Fatima Rana; Tahreem Tanweer; Amna Batool; Thomas J. Webster; Farid Menaa; Sundus Riaz; Zahra Rehman; Farhat Batool; Misha Fatima; Tuba Maryam; Iqra Shafique; Abida Saleem; Arfa Iqbal

doi:10.3390/nano11113152

Nanomaterials (Nov 2021)

Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Afrah Nawaz,
Syed Mohsin Ali,
Nosheen Fatima Rana,
Tahreem Tanweer,
Amna Batool,
Thomas J. Webster,
Farid Menaa,
Sundus Riaz,
Zahra Rehman,
Farhat Batool,
Misha Fatima,
Tuba Maryam,
Iqra Shafique,
Abida Saleem,
Arfa Iqbal

Affiliations

Afrah Nawaz: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Syed Mohsin Ali: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Nosheen Fatima Rana: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Tahreem Tanweer: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Amna Batool: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Thomas J. Webster: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA
Farid Menaa: Departments of Internal Medicine and Nanomedicine, California Innovations Corporation, San Diego, CA 92037, USA
Sundus Riaz: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Zahra Rehman: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Farhat Batool: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Misha Fatima: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Tuba Maryam: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Iqra Shafique: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Abida Saleem: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan
Arfa Iqbal: Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan

DOI: https://doi.org/10.3390/nano11113152
Journal volume & issue: Vol. 11, no. 11
p. 3152

Abstract

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Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of Enterococcus faecalis in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against E. faecalis compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating E. faecalis from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for E.-faecalis-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.

Published in Nanomaterials

ISSN: 2079-4991 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: https://www.mdpi.com/journal/nanomaterials

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