Frontiers in Molecular Neuroscience (Mar 2022)
Pseudo-Temporal Analysis of Single-Cell RNA Sequencing Reveals Trans-Differentiation Potential of Greater Epithelial Ridge Cells Into Hair Cells During Postnatal Development of Cochlea in Rats
- Jianyong Chen,
- Jianyong Chen,
- Jianyong Chen,
- Dekun Gao,
- Dekun Gao,
- Dekun Gao,
- Junmin Chen,
- Junmin Chen,
- Junmin Chen,
- Shule Hou,
- Shule Hou,
- Shule Hou,
- Baihui He,
- Baihui He,
- Baihui He,
- Yue Li,
- Yue Li,
- Yue Li,
- Shuna Li,
- Shuna Li,
- Shuna Li,
- Fan Zhang,
- Fan Zhang,
- Fan Zhang,
- Xiayu Sun,
- Xiayu Sun,
- Xiayu Sun,
- Yulian Jin,
- Yulian Jin,
- Yulian Jin,
- Lianhua Sun,
- Lianhua Sun,
- Lianhua Sun,
- Jun Yang,
- Jun Yang,
- Jun Yang
Affiliations
- Jianyong Chen
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Jianyong Chen
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Jianyong Chen
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Dekun Gao
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Dekun Gao
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Dekun Gao
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Junmin Chen
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Junmin Chen
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Junmin Chen
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Shule Hou
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Shule Hou
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Shule Hou
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Baihui He
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Baihui He
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Baihui He
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Yue Li
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Yue Li
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Yue Li
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Shuna Li
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Shuna Li
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Shuna Li
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Fan Zhang
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Fan Zhang
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Fan Zhang
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Xiayu Sun
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xiayu Sun
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Xiayu Sun
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Yulian Jin
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Yulian Jin
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Yulian Jin
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Lianhua Sun
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Lianhua Sun
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Lianhua Sun
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- Jun Yang
- Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Jun Yang
- Institute of Ear Science, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
- Jun Yang
- Shanghai Key Laboratory of Otolaryngology and Translational Medicine, Shanghai, China
- DOI
- https://doi.org/10.3389/fnmol.2022.832813
- Journal volume & issue
-
Vol. 15
Abstract
The hair cells of the cochlea play a decisive role in the process of hearing damage and recovery, yet knowledge of their regeneration process is still limited. Greater epithelial ridge (GER) cells, a type of cell present during cochlear development that has the characteristics of a precursor sensory cell, disappear at the time of maturation of hearing development. Its development and evolution remain mysterious for many years. Here, we performed single-cell RNA sequencing to profile the gene expression landscapes of rats’ cochlear basal membrane from P1, P7, and P14 and identified eight major subtypes of GER cells. Furthermore, single-cell trajectory analysis for GER cells and hair cells indicated that among the different subtypes of GER, four subtypes had transient cell proliferation after birth and could transdifferentiate into inner and outer hair cells, and two of them mainly transdifferentiated into inner hair cells. The other two subtypes eventually transdifferentiate into outer hair cells. Our study lays the groundwork for elucidating the mechanisms of the key regulatory genes and signaling pathways in the trans-differentiation of GER cell subtypes into hair cells and provides potential clues to understand hair cell regeneration.
Keywords
- sc-RNA sequencing
- pseudo-temporal analysis
- cochlear basal membrane
- greater epithelial ridge cells
- hair cells
