Rapid and Sensitive Electrochemical Assay of Cefditoren with MWCNT/Chitosan NCs/Fe₂O₃ as a Nanosensor

Abstract

In this research, a glassy carbon electrode (GCE) modified by MWCNT/chitosan NCs/Fe2O3 was prepared for the determination of the cephalosporin antibiotic cefditoren (CFT) using adsorptive stripping differential pulse and cyclic voltammetry techniques. The effects of pH, the scan rate, the deposition potential, the accumulation time, and modification agents on the determination of CFT were analyzed. The results showed that the modified electrode significantly increased the oxidation peak current of CFT. Under optimized conditions, the MWCNT/chitosan NCs/Fe2O3/GCE nanosensor exhibited a linear response between 0.2 µM and 10 µM toward CFT. The limit of detection and quantification were determined to be 1.65 nM and 5.50 nM, respectively. Model drugs (cefdinir, cefpodoxime, cephalexin, and ceftazidime compounds) were used to enlighten the CFT oxidation mechanism. Moreover, the nanosensor was used to analyze CFT in a pharmaceutical dosage form and commercial deproteinated human serum samples. The accuracy of these methods was proven in the recovery studies, with values of 96.98 and 98.62% for the pharmaceutical dosage form and commercial deproteinated human serum sample, respectively.

Keywords

• cefditoren
• cephalosporins
• antibiotics
• β-lactamases
• determination
• electroanalytical methods