Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment

Abstract

Objectives: The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods: Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated. Results: Enzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711 ± 29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50 °C and the molecular weight was 32 kDa. Conclusions: From these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent.

Keywords

• Fibrinolytic enzyme
• Bacillus
• Cheap substrates
• Blood clot
• Fibrinolytic agent