Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor

Wenxin Zhang; Yishi Hua; Weihao Di; Jinghui Guo; Qiuting Dai; Jian Zhao; Bei Zhang; Ying Wang

Stem Cell Research (Mar 2021)

Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor

Wenxin Zhang,
Yishi Hua,
Weihao Di,
Jinghui Guo,
Qiuting Dai,
Jian Zhao,
Bei Zhang,
Ying Wang

Affiliations

Wenxin Zhang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Yishi Hua: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China
Weihao Di: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jinghui Guo: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Qiuting Dai: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Jian Zhao: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China
Bei Zhang: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding authors.
Ying Wang: Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.

Journal volume & issue: Vol. 51
p. 102173

Abstract

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Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs and their ability to differentiate spontaneously three germ layers in vitro. The iPSC line displayed a normal karyotype. Our model offers the possibility to be used a control in pathological mechanism studies.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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