USAGE OF MUSHROOM AGARICUS BISPORUS TYROSINASE FOR SEPARATION OF PHENOLIC COMPOUNDS MIXTURES

Abstract

The new method of phenolic compounds mixtures separation in solutions was developed, based on the oxidation of one of the mixture component using immobilized in PVC partially purified tyrosinase and the next removal of the oxidation products with a help of potassium alum. The biocatalyst obtained had carried out oxidation of phenol or pyrocatechol (0,5-10 mmol/dm3) with quantitative degree of bioconversion in the presence of resorcinol, hydroquinone or p-nitrophenol, which are not oxidized, with reservation of their initial concentrations (35-65 mmol/dm3) in solution, during 4 cycles in batch reactor

Keywords

• тирозиназа, розділення сумішей, фенольні сполуки, іммобілізація