Time-resolved β-lactam cleavage by L1 metallo-β-lactamase

M. Wilamowski; D. A. Sherrell; Y. Kim; A. Lavens; R. W. Henning; K. Lazarski; A. Shigemoto; M. Endres; N. Maltseva; G. Babnigg; S. C. Burdette; V. Srajer; A. Joachimiak

doi:10.1038/s41467-022-35029-3

Nature Communications (Nov 2022)

Time-resolved β-lactam cleavage by L1 metallo-β-lactamase

M. Wilamowski,
D. A. Sherrell,
Y. Kim,
A. Lavens,
R. W. Henning,
K. Lazarski,
A. Shigemoto,
M. Endres,
N. Maltseva,
G. Babnigg,
S. C. Burdette,
V. Srajer,
A. Joachimiak

Affiliations

M. Wilamowski: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago
D. A. Sherrell: Structural Biology Center, X-ray Science Division, Argonne National Laboratory
Y. Kim: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago
A. Lavens: Structural Biology Center, X-ray Science Division, Argonne National Laboratory
R. W. Henning: Center for Advanced Radiation Sources, University of Chicago
K. Lazarski: Structural Biology Center, X-ray Science Division, Argonne National Laboratory
A. Shigemoto: Department of Chemistry and Biochemistry, Worcester Polytechnic Institute
M. Endres: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago
N. Maltseva: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago
G. Babnigg: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago
S. C. Burdette: Department of Chemistry and Biochemistry, Worcester Polytechnic Institute
V. Srajer: Center for Advanced Radiation Sources, University of Chicago
A. Joachimiak: Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago

DOI: https://doi.org/10.1038/s41467-022-35029-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

Read online

Metallo-β-lactamases cleave β-lactam moiety of many broadly used antibiotics. Here the authors captured mechanistic details of the enzyme catalyzed reaction using time-resolved xray synchrotron serial crystallography.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal