Ciencias Marinas (Mar 2004)

Hormone induced spawning (HCG), and embryonic and larval development of the leopard grouper, Mycteroperca rosacea (Streets, 1877)

  • V Gracia-López,
  • J Rodríguez-Romero,
  • JM Pérez-Ramírez

DOI
https://doi.org/10.7773/cm.v30i2.246
Journal volume & issue
Vol. 30, no. 2

Abstract

Read online

Induced spawning of two wild females of the leopard grouper, Mycteroperca rosacea, was obtained using Human Chorionic Gonadotropin (HCG) supplied in two dosages: a first injection of 1000 IU/kg and a second of 500 IU/kg, 24 h apart. Of the four treated females, two had a positive spawning response, releasing approximately 40,000 eggs/fish. These were fertilized with mature male sperm. The fertilization rate obtained for each female was 10% and 0%. Complete embryonic development, and the morphology of eggs and larvae are described until the first feeding. Eggs were spherical, buoyant and transparent, with a diameter of 872.8 ±15.4 ∞m (ranging from 850.4 to 912.5 ∞m) and an oil droplet diameter of 177.3 ± 7.0 ∞m.

Keywords