Solid-phase synthesis of negatively charged oligomeric polyamide mimetics of nucleic acids

Abstract

A Boc-protocol for manual solid-phase synthesis of novel sequences of negatively charged oligomeric polyamide mimetics of nucleic acids with a regular structure of the pseudopeptide backbone based on L-glutamic acid and glycine and having various positions of the carbethoxyethyl moiety was developed. Conditions for their cleavage from polymeric carrier, isolation and structure confirmation are presented.

Keywords

• peptide nucleic acids
• polyamide mimetics of nucleic acids
• solid-phase synthesis
• boc-protocol