Absolute quantification of a plasma tRNA-derived fragment for the diagnosis and prognosis of gastric cancer

Shuangshuang Zhang; Shuangshuang Zhang; Yaoyao Xie; Xiuchong Yu; Jiaxin Ge; Guoliang Ye; Guoliang Ye; Junming Guo; Junming Guo; Junming Guo

doi:10.3389/fonc.2023.1106997

Frontiers in Oncology (Apr 2023)

Absolute quantification of a plasma tRNA-derived fragment for the diagnosis and prognosis of gastric cancer

Shuangshuang Zhang,
Shuangshuang Zhang,
Yaoyao Xie,
Xiuchong Yu,
Jiaxin Ge,
Guoliang Ye,
Guoliang Ye,
Junming Guo,
Junming Guo,
Junming Guo

Affiliations

Shuangshuang Zhang: Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, China
Shuangshuang Zhang: Department of Gastroenterology, The Affiliated No. 1 Hospital, Ningbo University, Ningbo, China
Yaoyao Xie: Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, China
Xiuchong Yu: Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, China
Jiaxin Ge: Department of Gastroenterology, The Affiliated No. 1 Hospital, Ningbo University, Ningbo, China
Guoliang Ye: Department of Gastroenterology, The Affiliated No. 1 Hospital, Ningbo University, Ningbo, China
Guoliang Ye: Institute of Digestive Diseases of Ningbo University, Ningbo, China
Junming Guo: Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo, China
Junming Guo: Department of Gastroenterology, The Affiliated No. 1 Hospital, Ningbo University, Ningbo, China
Junming Guo: Institute of Digestive Diseases of Ningbo University, Ningbo, China

DOI: https://doi.org/10.3389/fonc.2023.1106997
Journal volume & issue: Vol. 13

Abstract

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BackgroundThe transition from a healthy gastric mucosa to gastric cancer is a multi-step process. Early screening can significantly improve the survival rate of gastric cancer patients. A reliable liquid biopsy for gastric cancer prediction is urgently needed and since tRNA-derived fragments (tRFs) are abundant in various body fluids, tRFs are possible new biomarkers for gastric cancer.MethodsA total of 438 plasma samples from patients with different gastric mucosal lesions as well as healthy individuals were collected. A specific reverse transcription primer, a forward primer, a reverse primer, and a TaqMan probe were designed. A standard curve was constructed and an absolute quantitation method was devised for detection of tRF-33-P4R8YP9LON4VDP in plasma samples of individuals with differing gastric mucosa lesions. Receiver operating characteristic curves were constructed to evaluate the diagnostic values of tRF-33-P4R8YP9LON4VDP for individual with differing gastric mucosa. A Kaplan–Meier curve was established to calculate the prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients. Finally, a multivariate Cox regression analysis was performed to assess the independent prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients.ResultsA detection method for plasma tRF-33-P4R8YP9LON4VDP was successfully established. Levels of plasma tRF-33-P4R8YP9LON4VDP were shown to reflect a gradient change from healthy individuals to gastritis patients to early and advanced gastric cancer patients. Significant differences were found among individuals with differing gastric mucosa, with reduced levels of tRF-33-P4R8YP9LON4VDP significantly related to a poor prognosis. tRF-33-P4R8YP9LON4VDP was found to be an independent predictor of an unfavorable survival outcome.ConclusionsIn this study, we developed a quantitative detection method for plasma tRF-33-P4R8YP9LON4VDP that exhibited hypersensitivity, convenience, and specificity. Detection of tRF-33-P4R8YP9LON4VDP was found to be a valuable means by which to monitor different gastric mucosa and to predict patient prognosis.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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