Genome-Wide Association Study of Nucleotide Variants Associated with Resistance to Nine Antimicrobials in <i>Mycoplasma bovis</i>

Abstract

Antimicrobial resistance (AMR) studies of Mycoplasma bovis have generally focused on specific loci versus using a genome-wide association study (GWAS) approach. A GWAS approach, using two different models, was applied to 194 Mycoplasma bovis genomes. Both a fixed effects linear model (FEM) and a linear mixed model (LMM) identified associations between nucleotide variants (NVs) and antimicrobial susceptibility testing (AST) phenotypes. The AMR phenotypes represented fluoroquinolones, tetracyclines, phenicols, and macrolides. Both models identified known and novel NVs associated (Bonferroni adjusted p M. bovis. This study was the largest collection of North American M. bovis isolates used with a GWAS for the sole purpose of identifying novel and non-antimicrobial-target NVs associated with AMR.

Keywords

• <i>Mycoplasma bovis</i>
• genome-wide association study
• antimicrobial resistance
• fluoroquinolone
• tetracycline
• phenicol