The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates

Gang Chen; Salma Mostafa; Zhaogeng Lu; Ran Du; Jiawen Cui; Yun Wang; Qinggang Liao; Jinkai Lu; Xinyu Mao; Bang Chang; Quan Gan; Li Wang; Zhichao Jia; Xiulian Yang; Yingfang Zhu; Jianbin Yan; Biao Jin

Genomics, Proteomics & Bioinformatics (Feb 2023)

The Jasmine (Jasminum sambac) Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates

Gang Chen,
Salma Mostafa,
Zhaogeng Lu,
Ran Du,
Jiawen Cui,
Yun Wang,
Qinggang Liao,
Jinkai Lu,
Xinyu Mao,
Bang Chang,
Quan Gan,
Li Wang,
Zhichao Jia,
Xiulian Yang,
Yingfang Zhu,
Jianbin Yan,
Biao Jin

Affiliations

Gang Chen: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
Salma Mostafa: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Department of Floriculture, Faculty of Agriculture, Alexandria University, Alexandria 21526, Egypt
Zhaogeng Lu: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
Ran Du: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Jiawen Cui: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Yun Wang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Qinggang Liao: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Jinkai Lu: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Xinyu Mao: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Bang Chang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Quan Gan: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Li Wang: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Zhichao Jia: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Xiulian Yang: College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
Yingfang Zhu: Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, Kaifeng 475001, China
Jianbin Yan: Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; Corresponding authors.
Biao Jin: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China; Corresponding authors.

Journal volume & issue: Vol. 21, no. 1
pp. 127 – 149

Abstract

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Jasminum sambac (jasmine flower), a world-renowned plant appreciated for its exceptional flower fragrance, is of cultural and economic importance. However, the genetic basis of its fragrance is largely unknown. Here, we present the first de novo genome assembly of J. sambac with 550.12 Mb (scaffold N50 = 40.10 Mb) assembled into 13 pseudochromosomes. Terpene synthase (TPS) genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome. Gene clusters within the salicylic acid/benzoic acid/theobromine (SABATH) and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase (BAHD) superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds. Several key genes involved in jasmonate biosynthesis were duplicated, causing an increase in copy numbers. In addition, multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways. Furthermore, the roles of JsTPS3 in β-ocimene biosynthesis, as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis, were functionally validated. The genome assembled in this study for J. sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis, and provides a foundation for functional genomic research and variety improvements in Jasminum.

Published in Genomics, Proteomics & Bioinformatics

ISSN: 1672-0229 (Print); 2210-3244 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://academic.oup.com/gpb

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