The complete mitogenome of Toxascaris leonina from the Siberian tiger (Panthera tigris altaica)

Abstract

Toxascaris leonina is a polyxenical parasite and commonly found in canids and felids. In this study, we used the Illumina high throughput sequencing and assembly to determine the complete mitogenome of a representative of this parasite from the Siberian tiger (Panthera tigris altaica). The genome was 14,248 bp in size and encoded 12 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs. Phylogeny showed that two canid (dog)-originated T. leonina were phylogenetically distinct from two felid-originated T. leonina (tiger isolate and cheetah isolate), suggesting at least two distinct subclades of T. leonina infecting these hosts and supporting once again that T. leonina represents a species complex. Furthermore, four isolates of T. leonina grouped together and were more closely related to other species from the family Ascarididae than species of families Toxocaridae, Anisakidae and Ascaridiidae, demonstrating phylogenetic stability of these paraphyletic groups characterized in this study. These cumulative mitochondrial DNA data provide a better understanding of phylogenetic relationships of this polyxenical and zoonotic roundworm species.

Keywords

• toxascaris leonina
• tiger roundworm
• mitochondrial genome
• phylogenetic relationship