Biosynthesis of silver nanoparticles using curcumin against the bovine mastitis bacteria

Abstract

Bovine mastitis is the most common disease and has greatly affected economies around the world. This study aimed to determine the antibacterial ability of bovine mastitis by curcumin - silver nanoparticles (Cur-AgNPs). The study included experiments on presenting a new process for synthesizing silver nanoparticles using curcumin from fresh turmeric as a reducing agent and stabilizer. UV-visible spectroscopy of the samples revealed the localized surface plasmon resonance absorbance of the dispersion of silver nanoparticles at 430 nm. The prepared Cur-AgNPs has a spherical shape with an average size of 30 nm and a size distribution of 15–47 nm. FT-IR (Fourier transform infrared spectroscopy) measurements of the samples showed that silver nanopartilces has been encapsulated well by curcumin. Cur-AgNPs with a concentration of 50-200 μg/mL has very effect to Staphylococcus aureus, Pseudomonas aeruginosa causing bovine mastitis in vitro. The maximum inhibition zone formed was 15 ± 0.85 mm for Staphylococcus aureus and 14 ± 0.56 mm for Pseudomonas aeruginosa. Plant materials mediating for the synthesis of silver nanoparticles have relatively rapid, less expensive, and widespread applications for antimicrobial therapy in the livestock sector.

Keywords

• antibacterial activity
• curcumin
• mastitis causing bacteria
• silver nanoparticles