Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma

Xiaoli Wu; Xiaoqing Meng; Tse-Shao Chang; Shuo Feng; Miki Lee; Sangeeta Jaiswal; Eun-Young K. Choi; Lam Tran; Hui Jiang; Thomas D. Wang

Photoacoustics (Jun 2022)

Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma

Xiaoli Wu,
Xiaoqing Meng,
Tse-Shao Chang,
Shuo Feng,
Miki Lee,
Sangeeta Jaiswal,
Eun-Young K. Choi,
Lam Tran,
Hui Jiang,
Thomas D. Wang

Affiliations

Xiaoli Wu: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA
Xiaoqing Meng: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA
Tse-Shao Chang: Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Shuo Feng: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA
Miki Lee: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA
Sangeeta Jaiswal: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA
Eun-Young K. Choi: Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
Lam Tran: Department of Biostatistics, University of Michigan, Ann Arbor, MI 48109, USA
Hui Jiang: Department of Biostatistics, University of Michigan, Ann Arbor, MI 48109, USA
Thomas D. Wang: Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Correspondence to: Biomedical Engineering, and Mechanical Engineering Division of Gastroenterology, University of Michigan, 109 Zina Pitcher Pl. BSRB 1522, Ann Arbor, MI 48109-2200, USA.

Journal volume & issue: Vol. 26
p. 100355

Abstract

Read online

Background: Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early cancer detection and image-guided surgery. Methods: We used a structural model to optimize the peptide sequence. Specific binding was validated in vitro with knockdown, competition, and co-localization assays. Multi-modal imaging was performed to validate specific binding in vivo in orthotopically-implanted human xenograft tumors. Results: Binding properties of WKGWSYLWTQQA were characterized by an apparent dissociation constant of kd = 43 nM, and an apparent association time constant of k = 0.26 min−1. The target-to-background ratio was significantly higher for the target versus control for both modalities. Ex-vivo evaluation using human HCC specimens supported the ability of the peptide to distinguish HCC from other liver pathologies. Conclusions: We have identified a peptide specific for CD44 with properties that are promising for clinical translation to image HCC in vivo.

Published in Photoacoustics

ISSN: 2213-5979 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Physics: Acoustics. Sound; Science: Physics: Optics. Light
Website: http://www.journals.elsevier.com/photoacoustics/

About the journal

Abstract

Keywords