Nature Communications (Dec 2022)

High-throughput robust single-cell DNA methylation profiling with sciMETv2

  • Ruth V. Nichols,
  • Brendan L. O’Connell,
  • Ryan M. Mulqueen,
  • Jerushah Thomas,
  • Ashley R. Woodfin,
  • Sonia Acharya,
  • Gail Mandel,
  • Dmitry Pokholok,
  • Frank J. Steemers,
  • Andrew C. Adey

DOI
https://doi.org/10.1038/s41467-022-35374-3
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 10

Abstract

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Despite the importance of DNA methylation, accessible and high-throughput methods to profile methylation at the single-cell level are lacking. Here, the authors present sciMETv2, a high-throughput workflow that provides high-quality single-cell methylomes in a robust and simple workflow.