Frontiers in Physiology (May 2019)

Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells

  • Jian-Feng Qiu,
  • Jian-Feng Qiu,
  • Xue Li,
  • Xue Li,
  • Wen-Zhao Cui,
  • Wen-Zhao Cui,
  • Xiao-Fei Liu,
  • Xiao-Fei Liu,
  • Hui Tao,
  • Hui Tao,
  • Kun Yang,
  • Kun Yang,
  • Tai-Ming Dai,
  • Tai-Ming Dai,
  • Yang-Hu Sima,
  • Yang-Hu Sima,
  • Shi-Qing Xu,
  • Shi-Qing Xu

DOI
https://doi.org/10.3389/fphys.2019.00537
Journal volume & issue
Vol. 10

Abstract

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Circadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a Bombyx mori animal model of single Period gene (BmPer) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a B. mori ovarian cell line (BmN) by continuous knock down of BmPer expression. The effects of BmPer expression knockdown (Per-KD) on cell proliferation and apoptosis were opposite to those of m/hPer1 and m/hPer2 in mammals. The knockdown of BmPer expression led to cell cycle deceleration with shrinking of the BmN cell nucleus, and significant inhibition of nuclear DNA synthesis and cell proliferation. It also promoted autophagy via the lysosomal pathway, and accelerated apoptosis via the caspase pathway.

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