Research Note: Polymorphisms of gonadotrophin-releasing hormone gene and their association with growth traits in quail (Coturnix Coturnix)

Xinle Wang; Jingyun Li; Junyan Bai; Longwei Wang; Hongdeng Fan; Mengke Chen; Fanlin Zeng; Xiaoning Lu; Yuhan He

Poultry Science (Mar 2023)

Research Note: Polymorphisms of gonadotrophin-releasing hormone gene and their association with growth traits in quail (Coturnix Coturnix)

Xinle Wang,
Jingyun Li,
Junyan Bai,
Longwei Wang,
Hongdeng Fan,
Mengke Chen,
Fanlin Zeng,
Xiaoning Lu,
Yuhan He

Affiliations

Xinle Wang: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Jingyun Li: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Junyan Bai: Corresponding author:; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Longwei Wang: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Hongdeng Fan: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Mengke Chen: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Fanlin Zeng: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Xiaoning Lu: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China
Yuhan He: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471003, China

Journal volume & issue: Vol. 102, no. 3
p. 102439

Abstract

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ABSTRACT: This study aimed to identify polymorphisms of gonadotropin-releasing hormone (GnRH) gene and their association with growth traits in quail by PCR and direct sequencing. Genomic DNA was extracted from quail blood samples of 36 from Savimalt (SV) and 49 from French Giant (FG). Growth traits were measured and used for candidate gene analysis, as body weight (BW), shank length (SL), chest width (CW), chest depth (CD), breastbone length (BBL), body length (BL), and shank circumference (SC). The results showed that a total of 20 SNPs were detected in GnRH gene, whereas 8 SNPs were significantly associated with growth traits (P < 0.05). The T215C, G279A, C458T, A520G, and C547G were significantly associated with SL at 3 wk of age in the FG strain, whereas A583T was significantly related to BBL and BL, and C591T was significantly related to SL, BBL, and BL, whereas A592G was significantly correlated with SL, CW, CD, BBL, and BL (P < 0.05). The 8 SNPs were significantly related to CW, CD, and BBL at 3 wk of age in the SV strain, whereas A583T, C591T, and A592G were significantly associated with BW (P < 0.05). The G279A showed significant correlations with SL at 5 wk of age in FG, whereas A583T showed significant associations with SC in FG, and C591T was significantly associated with BW and SC in FG, whereas A592T was significantly related to BW, SL, and CD in FG (P < 0.05). The T215C, G279A, C458T, A520G, and C547G were significantly correlated with BW, CW, BBL, and BL at 5 wk of age in SV, whereas A583T, C591T, and A592G were significantly related to BW, SL, CW, BBL, and BL (P < 0.05). Haplotypes based on 8 SNPs showed significant correlation with BW, SL, CW, CD, BBL, BL, and SC in FG (P < 0.05). In conclusion, the GnRH gene could be used as a molecular genetic marker to provide theoretical foundation to improve growth traits in quail.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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