Identification of Staphylococcus Aureus Enterotoxin Genes Using Multiplex PCR

Abstract

BACKGROUND AND OBJECTIVE: Staphylococcus aureus is one of the most common causes of food poisoning in the world. Various studies indicated that 18-15 percent aureus strains isolated from different sources are be able to produce enterotoxin which known as the main factor of poisoning. The aim of this study was to identify enterotoxin genes, such as: (femA, see, sed, sec, seg, seb, sea) in S. aureus confirmed using multiple PCR method. METHODS: In this study, 60 samples of Staphylococcus aureus isolated from purulent infections, skin and have symptoms of poisoning: vomiting and diarrhea in humans, production of enterotoxin was examined. After DNA extraction of isolates, multiple PCR using specific primers for enterotoxin genes was performed. FINDINGS: Overall, 50% of Staphylococcus aureus isolates contain one or more enterotoxin gene. The most abundant gene was sea (30%) and sed (10%), see (3/8%), sec (6/1%) were also identified. CONCLUSION: It was found that other genes such as TSST-1 are involved staphylococcus aureus enterotoxin production in the creation of visual acuity in addition to above genes. In general, the presence of Staphylococcus aureus in human clinical specimens, especially enterotoxigenic strains, can be considered a potential risk for health

Keywords

• Staphylococcus aureus
• enterotoxin gene
• multiplex polymerase chain reaction
• food poisoning