Frontiers in Plant Science (Oct 2019)

Negative Regulation of Age-Related Developmental Leaf Senescence by the IAOx Pathway, PEN1, and PEN3

  • Renee A. Crane,
  • Marielle Cardénas Valdez,
  • Nelly Castaneda,
  • Charidan L. Jackson,
  • Ciairra J. Riley,
  • Islam Mostafa,
  • Islam Mostafa,
  • Wenwen Kong,
  • Shweta Chhajed,
  • Sixue Chen,
  • Sixue Chen,
  • Sixue Chen,
  • Sixue Chen,
  • Judy A. Brusslan

DOI
https://doi.org/10.3389/fpls.2019.01202
Journal volume & issue
Vol. 10

Abstract

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Early age-related developmental senescence was observed in Arabidopsis cyp79B2/cyp79B3 double mutants that cannot produce indole-3-acetaldoxime (IAOx), the precursor to indole glucosinolates (IGs), camalexin and auxin. The early senescence phenotype was not observed when senescence was induced by darkness. The cyp79B2/cyp79B3 mutants had lower auxin levels, but did not display auxin-deficient phenotypes. Camalexin biosynthesis mutants senesced normally; however, IG transport and exosome-related pen1/pen3 double mutants displayed early senescence. The early senescence in pen1/pen3 mutants depended on salicylic acid and was not observed in pen1 or pen3 single mutants. Quantitation of IGs showed reduced levels in cyp79B2/cyp79B3 mutants, but unchanged levels in pen1/pen3, even though both of these double mutants display early senescence. We discuss how these genetic data provide evidence that IAOx metabolites are playing a protective role in leaf senescence that is dependent on proper trafficking by PEN1 and PEN3, perhaps via the formation of exosomes.

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