Negative Regulation of Age-Related Developmental Leaf Senescence by the IAOx Pathway, PEN1, and PEN3

Renee A. Crane; Marielle Cardénas Valdez; Nelly Castaneda; Charidan L. Jackson; Ciairra J. Riley; Islam Mostafa; Islam Mostafa; Wenwen Kong; Shweta Chhajed; Sixue Chen; Sixue Chen; Sixue Chen; Sixue Chen; Judy A. Brusslan

doi:10.3389/fpls.2019.01202

Frontiers in Plant Science (Oct 2019)

Negative Regulation of Age-Related Developmental Leaf Senescence by the IAOx Pathway, PEN1, and PEN3

Renee A. Crane,
Marielle Cardénas Valdez,
Nelly Castaneda,
Charidan L. Jackson,
Ciairra J. Riley,
Islam Mostafa,
Islam Mostafa,
Wenwen Kong,
Shweta Chhajed,
Sixue Chen,
Sixue Chen,
Sixue Chen,
Sixue Chen,
Judy A. Brusslan

Affiliations

Renee A. Crane: Milken School, Los Angeles, CA, United States
Marielle Cardénas Valdez: Department of Biological Sciences, California State University, Long Beach, Long Beach, CA, United States
Nelly Castaneda: Department of Biological Sciences, California State University, Long Beach, Long Beach, CA, United States
Charidan L. Jackson: Department of Biological Sciences, California State University, Long Beach, Long Beach, CA, United States
Ciairra J. Riley: Department of Biological Sciences, California State University, Long Beach, Long Beach, CA, United States
Islam Mostafa: Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, United States
Islam Mostafa: Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
Wenwen Kong: Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, United States
Shweta Chhajed: Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, United States
Sixue Chen: Department of Biology, Genetics Institute, University of Florida, Gainesville, FL, United States
Sixue Chen: Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
Sixue Chen: Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, United States
Sixue Chen: Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, United States
Judy A. Brusslan: Department of Biological Sciences, California State University, Long Beach, Long Beach, CA, United States

DOI: https://doi.org/10.3389/fpls.2019.01202
Journal volume & issue: Vol. 10

Abstract

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Early age-related developmental senescence was observed in Arabidopsis cyp79B2/cyp79B3 double mutants that cannot produce indole-3-acetaldoxime (IAOx), the precursor to indole glucosinolates (IGs), camalexin and auxin. The early senescence phenotype was not observed when senescence was induced by darkness. The cyp79B2/cyp79B3 mutants had lower auxin levels, but did not display auxin-deficient phenotypes. Camalexin biosynthesis mutants senesced normally; however, IG transport and exosome-related pen1/pen3 double mutants displayed early senescence. The early senescence in pen1/pen3 mutants depended on salicylic acid and was not observed in pen1 or pen3 single mutants. Quantitation of IGs showed reduced levels in cyp79B2/cyp79B3 mutants, but unchanged levels in pen1/pen3, even though both of these double mutants display early senescence. We discuss how these genetic data provide evidence that IAOx metabolites are playing a protective role in leaf senescence that is dependent on proper trafficking by PEN1 and PEN3, perhaps via the formation of exosomes.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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