Metabolomics Study on the Differences of Endogenous Small Molecule 
between A549/DDP and A549 Cells Based on High Solution UPLC-TOF-MS

Abstract

Background and objective Cisplatin acquired resistance is a vital problem in the chemotherapy of non-small cell lung cancer, which needs to be further addressed. In recent years, obtaining drug resistant cells from cell cultivation and serving for metabolomics research to find differential metabolites and get potential biomarkers, is a good reference for clinical research and cancer treatment. This study aimed to obtain metabolite information related to cisplatin resistance through metabolomics analysis. Methods Metabolites were extracted from A549 cells and cisplatin resistant A549/DDP cells, and ultraperformance liquid chromatography coupled with time of flight mass spectrometry was used to perform metabolomic analysis of endogenous molecules of the two cells and obtain metabolic differences. Results Through data analysis, 40 metabolites were identified as differential metabolites, mainly involving phospholipids, fatty acids, amino acids and metabolites related to energy metabolism. Conclusion The drug resistance of A549/DDP cells may be caused by the changes of cell membrane structure and related metabolic pathways.

Keywords

• Lung neoplasms
• Chemoresistance
• Cisplatin
• Metabolomics