Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing

Yuanyuan Dai; Li Chen; Wenjiao Chang; Huaiwei Lu; Peng Cui; Xiaoling Ma

doi:10.3389/fpubh.2019.00379

Frontiers in Public Health (Dec 2019)

Culture-Negative Streptococcus suis Infection Diagnosed by Metagenomic Next-Generation Sequencing

Yuanyuan Dai,
Li Chen,
Wenjiao Chang,
Huaiwei Lu,
Peng Cui,
Xiaoling Ma

Affiliations

Yuanyuan Dai: Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China
Li Chen: Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China
Wenjiao Chang: Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China
Huaiwei Lu: Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China
Peng Cui: Infectious Disease Research and Development, Beijing Genomics Institute-Shanghai, Shanghai, China
Xiaoling Ma: Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China

DOI: https://doi.org/10.3389/fpubh.2019.00379
Journal volume & issue: Vol. 7

Abstract

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Background:Streptococcus suis is a zoonotic pathogen that can cause severe infections such as meningitis and septicemia in both swine and humans. Rapid and accurate identification of the causative agent is very important for guiding clinical choices in administering countermeasures.Case Report: Here, we report a case of fatal S. suis infection in a patient who worked as a butcher in China. The 59-year-old man, who had previously undergone splenectomy, injured his finger while processing pork and developed severe sepsis. While blood cultures were negative following antibiotic treatment, S. suis was determined to be the causative agent by metagenomic next-generation sequencing (mNGS) and Sanger sequencing.Conclusion: Identification of etiological agents using techniques such as blood culture prior to antibiotic treatment is very important. mNGS may represent a useful method for diagnosis of infectious diseases, especially post-antibiotic treatment.

Published in Frontiers in Public Health

ISSN: 2296-2565 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Public aspects of medicine
Website: https://www.frontiersin.org/journals/public-health

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