Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies

Tae-Eun Park; Nur Mustafaoglu; Anna Herland; Ryan Hasselkus; Robert Mannix; Edward A. FitzGerald; Rachelle Prantil-Baun; Alexander Watters; Olivier Henry; Maximilian Benz; Henry Sanchez; Heather J. McCrea; Liliana Christova Goumnerova; Hannah W. Song; Sean P. Palecek; Eric Shusta; Donald E. Ingber

doi:10.1038/s41467-019-10588-0

Nature Communications (Jun 2019)

Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies

Tae-Eun Park,
Nur Mustafaoglu,
Anna Herland,
Ryan Hasselkus,
Robert Mannix,
Edward A. FitzGerald,
Rachelle Prantil-Baun,
Alexander Watters,
Olivier Henry,
Maximilian Benz,
Henry Sanchez,
Heather J. McCrea,
Liliana Christova Goumnerova,
Hannah W. Song,
Sean P. Palecek,
Eric Shusta,
Donald E. Ingber

Affiliations

Tae-Eun Park: Wyss Institute for Biologically Inspired Engineering at Harvard University
Nur Mustafaoglu: Wyss Institute for Biologically Inspired Engineering at Harvard University
Anna Herland: Wyss Institute for Biologically Inspired Engineering at Harvard University
Ryan Hasselkus: Wyss Institute for Biologically Inspired Engineering at Harvard University
Robert Mannix: Wyss Institute for Biologically Inspired Engineering at Harvard University
Edward A. FitzGerald: Wyss Institute for Biologically Inspired Engineering at Harvard University
Rachelle Prantil-Baun: Wyss Institute for Biologically Inspired Engineering at Harvard University
Alexander Watters: Wyss Institute for Biologically Inspired Engineering at Harvard University
Olivier Henry: Wyss Institute for Biologically Inspired Engineering at Harvard University
Maximilian Benz: Wyss Institute for Biologically Inspired Engineering at Harvard University
Henry Sanchez: Wyss Institute for Biologically Inspired Engineering at Harvard University
Heather J. McCrea: Department of Neurosurgery, Boston Children’s Hospital and Harvard Medical School
Liliana Christova Goumnerova: Department of Neurosurgery, Boston Children’s Hospital and Harvard Medical School
Hannah W. Song: Department of Chemical and Biological Engineering, University of Wisconsin-Madison
Sean P. Palecek: Department of Chemical and Biological Engineering, University of Wisconsin-Madison
Eric Shusta: Department of Chemical and Biological Engineering, University of Wisconsin-Madison
Donald E. Ingber: Wyss Institute for Biologically Inspired Engineering at Harvard University

DOI: https://doi.org/10.1038/s41467-019-10588-0
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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In vitro blood-brain barrier (BBB) models do not fully recapitulate the in vivo barrier function. Here the authors develop an organ-on-a-chip BBB model using iPS-derived human brain endothelial cells differentiated under hypoxia, primary human pericytes and astrocytes, which maintains in vivo-like BBB barrier and shuttling functions for a week.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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