Scientific Reports (Nov 2020)

Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing

  • Norbert Moldován,
  • Gábor Torma,
  • Gábor Gulyás,
  • Ákos Hornyák,
  • Zoltán Zádori,
  • Victoria A. Jefferson,
  • Zsolt Csabai,
  • Miklós Boldogkői,
  • Dóra Tombácz,
  • Florencia Meyer,
  • Zsolt Boldogkői

DOI
https://doi.org/10.1038/s41598-020-77520-1
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 14

Abstract

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Abstract Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.