Evaluación de la esterilización húmeda y la esterilización por microondas de dos tipos de suelos

Abstract

In the present study the sterilization was evaluated in two types of soil, low microbial population and moisture (BB) and another with high micro- bial population and intermediate moisture (AM). The methods evaluated were autoclaving (121°C) and microwave irradiation (4 min). Three auto- claving treatments were made by three consecutive days and each evalua- tion varied in the time of sterilization and the temperature of incubation between the cycles of sterilization. For the soil AM was not obtained counts in autoclaving treatments (10-2) and the counts diminished in soil BB, but did not obtained sterilization by any of the methods evaluated. In all treatments in autoclave observed decrease in water content caused by the differential temperature of incubation used. The results showed that there is a relation between the moisture of the soil and the efficiency of the humid sterilization and in the sterilization with microwaves, relation that is different for the two methods.

Keywords

• soil sterilization
• loss moisture
• inhibition of microorganisms
• moist head sterilization
• microwaves.